mFISH based Immunotherapy statustracking
Techniques for analyzing the spatial organization of the tumor microenvironment(TME) and the immune cells within are lacking, as immunohistochemical methods are extremely low-throughput. Single-cell techniques, being unbiased in analyzing the variety and composition of cell types will be capable of precisely detecting disease-associated cell states, including rare but potent ones.
In this project, we aim to use multiplex error robust fluorescence-in-situ-hybridization (mFISH) to create accurate, sub-cellular-resolution cellular profiles of the colorectal TME and study the roles and interactions of different cell types. We will also compare the immune alterations between responders and non-responders to immunotherapy, and identify (spatial) biomarkers that may shed light on the mechanisms of immunotherapy responses.
